The regulation of pyruvate dehydrogenase in the isolated perfused rat heart.
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چکیده
منابع مشابه
The Regulation of Pyruvate Dehydrogenase in the Isolated Perfused Rat Heart*
The regulation of the pyruvate dehydrogenase multienzyme complex was studied in the isolated perfused rat heart. Because 95% of the “CO2 produced as a result of the decarboxylation of [l-‘4C]pyruvate occurs via pyruvate dehydrogenase in the heart, the rate of 14COz production from infused [l-‘4C]pyruvate was utilized as the monitor of the metabolic flux through this enzyme complex. In contrast ...
متن کاملStudies on the regulation of pyruvate dehydrogenase in the isolated perfused rat heart.
The regulation of the pyruvate dehydrogenase multienzyme complex was studied in the isolated perfused rat heart. Because 95% of the “CO2 produced as a result of the decarboxylation of [l-‘4C]pyruvate occurs via pyruvate dehydrogenase in the heart, the rate of 14COz production from infused [l-‘4C]pyruvate was utilized as the monitor of the metabolic flux through this enzyme complex. In contrast ...
متن کاملRegulation of Pyruvate Dehydrogenase in Rat Heart
1. The proportion of active (dephosphorylated) pyruvate dehydrogenase in perfused rat heart was decreased by alloxan-diabetes or by perfusion with media containing acetate, n-octanoate or palmitate. The total activity, of the dehydrogenase was unchanged. 2. Pyruvate (5 or 25mm) or dichloroacetate (1m) increased the proption of active (dephosphorylated) pyruvate debydrogenase in perfused rat hea...
متن کاملStudies of the effects of beta-adrenergic agonists on the regulation of pyruvate dehydrogenase in the perfused rat heart.
The effects of &adrenergic agonists (e.g. epinephrine and isoproterenol) on the metabolic flux through, and the activation state of the pyruvate dehydrogenase multienzyme complex, were investigated in the isolated perfused rat heart. Infusion of epinephrine caused an inhibition of pyruvate decarboxylation at low (0.1 m ~ ) pyruvate concentrations in the perfusion medium, a stimulation of flux a...
متن کاملAcyloin production from aldehydes in the perfused rat heart: the potential role of pyruvate dehydrogenase.
Aldehydes represent an important class of cytotoxic products derived from free radical-induced lipid peroxidation which may contribute to reperfusion injury following myocardial infarct. Metabolism of aldehydes in the heart has not been well characterized aside from conjugation of unsaturated aldehydes with glutathione. However, aliphatic aldehydes like hexanal do not form stable glutathione co...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)34510-6